小鼠Dickkopf相关蛋白3(DKK3)多克隆抗体
产品名称： 小鼠Dickkopf相关蛋白3(DKK3)多克隆抗体
英文名称： Polyclonal Antibody to Dickkopf Related Protein 3 (DKK3)
产品编号： YBD7410Mu01
产品价格： null
产品产地： 中国/上海
品牌商标： 钰博生物
更新时间： 2023-08-17T10:29:50
使用范围： WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
上海钰博生物科技有限公司
- 联系人 : 陈环环
- 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 183****2235 点击查看
- 传真 : 点击查看
- 邮箱 : shybio@126.com
- 二维码 : 点击查看
小鼠Dickkopf相关蛋白3(DKK3)多克隆抗体
Polyclonal Antibody to Dickkopf Related Protein 3 (DKK3)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
[ IMMUNOGEN ]
Immunogen: Recombinant DKK3 (Pro23~Ile349) expressed in E.coli.
[ APPLICATIONS ] Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant DKK3 disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ IDENTIFICATION ]
Figure 1. Western Blot
A. Sample: Recombinant DKK3, Mouse B. Mouse Serum
Primary Ab: 3µg/mL Rabbit Anti-Mouse DKK3 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。