GST GraviTrap-蛋白修饰-试剂-生物在线
上海基星生物科技有限公司
GST GraviTrap

GST GraviTrap

商家询价

产品名称: GST GraviTrap

英文名称: GST GraviTrap

产品编号: 28-9523-60

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
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  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
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28-4055-00 GST MULTITRAP 4B
28-4055-01 GST MULTITRAP FF
28-9523-59 GST SpinTrap
28-9523-60 GST GraviTrap
28-9523-61 GST Buffer Kit
28-9545-49 PGEX-4T-1
28-9545-50 PGEX-4T-2
28-9545-52 PGEX-4T-3
28-9545-53 PGEX-5X-1
28-9545-54 PGEX-5X-2
28-9545-55 PGEX-5X-3
28-9546-46 PGEX-2TK
28-9546-48 PGEX-6P-1 VECTOR
28-9546-50 PGEX-6P-2 VECTOR
28-9546-51 PGEX-6P-3 VECTOR
28-9546-53 PGEX-2T 25 UG WI/DI
28-9546-54 PGEX-3X 25 UG WI/DI
28-9546-56 PGEX-1LT ECOR I/BAP
11-0033-99 HIS GRAVITRAP
11-0034-00 HIS BUFFER KIT
27-4710-01 ANTI-HIS ANTIBODY
28-4009-89 HIS MULTITRAP HP
28-4009-90 HIS MULTITRAP FF
28-4013-53 HIS SPINTRAP
28-4039-43 COLLECTION PLATE
28-9321-71 His SpinTrap Kit
27-1583-01 LIGHT PRIMER MIX
27-1586-01 HEAVY PRIMER MIX
27-1588-01 LINKER-PRIMER MIX
27-1589-01 RS PRIMER MIX
27-9420-01 ANTI-M13 MONOCLONAL ANTIBODY
27-9421-01 HRP/ANTI-M13 MONOCLNL CONJUG.

详情请查询以下网址:

http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/zh/GELifeSciences-us/28952360

GST GraviTrap

GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein

  • Convenient, prepacked gravity-flow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure proteins per column.
  • Yields up to 20 mg of pure proteins per column

 

GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein. GST-tagged proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins.

 

The binding capacity of prepacked GST GraviTrap columns, which can be greater than 10 mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.

GST GraviTrap — Technical Specifications

Complete Packsize 10 x 2 ml
Bed Volume 2 ml
Binding Capacity/Column Up to 20 mg recombinant glutathione S-transferase (rGST)
Storage Conditions 4 to 30°C, 20% Ethanol
   
Media  
Ligand Glutathione
Average Particle Size 90 μm
Matrix Agarose, 4%
Particle Size 45 µm-165 µm
Binding Capacity/ml Chromatography Medium > 25 mg horse liver GST/ml medium1)
pH stability Working Range 4-13
pH stability Cleaning 4-13
Storage Conditions 4 to 30°C, 20% Ethanol
Chemical Stability All commonly used aqueous buffers
1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.  
   
Column  
Column i.d. 15 mm
Chemical Stability Stable in all commonly used buffer systems
Material [Column Hardware] Polypropylene (PP)
Material [Bed Support] Polyethylene (PE)