Synonyms:	EFM-192B
Background:	established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; cells were described in the literature to overexpress the erB-2 gene; sister cell lines EFM-192A and -192C show differences in molecular aspects
Species:	human (Homo sapiens)
Tissue:	breast
Disease:	breast carcinoma
Gender:	N/A
Morphology:	epitheloid adherent cells growing as monolayers
Growth Mode:	N/A
Doubling Time:	ca. 4-5 days
DNA Profile:	N/A  Cell Line Authentication Service
Culture Medium:	PMI-1640+20%FBS We strongly suggest to purchase the complete medium from .
Cryopreservation medium:	90%FBS+10%DMSO
Mycoplasma:	negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:	cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:	same DNA profile as EFM-192A/EFM-192C using multiplex PCR of minisatellite markers
Species:	confirmed as human with IEF of LDH, MDH, NP
Cytogenetics:	human heteroploid hypotriploid karyotype with 13% polyploidy - 62(58-62)<3n>XXX/XX, -X, +2, +4, +6, -7, -8, -8, -10, -11, -12, +14, +16, -17, -17, -17, -18, -18, -19, -20, -21, -22, +8mar, add(1)(p22-31), add(2)(q?35), del(3)(p22), add(5)(p15), del(6)(q25), add(10)(p11), add(13)(q32), add(14)(p12), add(15)(p12), der(15)t(10;15)(q11;p12), der(20)t(1;20)(q24;q12/13), der(21)t(7;21)(q11;p12)x2 - most cells carry a small ring and 1-2 large submetacentric 15p+ markers - closely related to EFM-192A - apparent ch 17 nullisomy
Virus	ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -