FITC标记的氨基肽酶1抗体
产品名称： FITC标记的氨基肽酶1抗体
英文名称： Anti-MetAP1/FITC
产品编号： HZ-18806R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
Rabbit Anti-MetAP1/FITC Conjugated antibody
FITC标记的氨基肽酶1抗体
| 英文名称 | Anti-MetAP1/FITC |
| 中文名称 | FITC标记的氨基肽酶1抗体 |
| 别 名 | Methionyl Aminopeptidase 1; AMPM1_HUMAN; DKFZp781C0419; KIAA0094; MAP 1; MAP1A; MetAP-1; MetAP1; metap1; MetAP1A; Methionine aminopeptidase 1; Peptidase M 1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 43kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MetAP1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: MetAP-1 is a 394-amino acid protein that is expressed at low levels in all tissues, but is highly expressed in skeletal muscles. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. The control of cell proliferation in mammalian cells is directly linked and strictly dependent on the evolutionarily highly conserved mechanism that MetAP-1 employs. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. Pyridine-2-carboxylic acid thiazol-2-ylamide (PCAT) forms a scaffold that inhibits the action of MetAP-1, while 1,2,4-triazol is a non-peptide inhibitor of MetAP-1 binding to the active site with the N1 and N2 atoms of the triazole moiety complexing two divalent ions. Function: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle. Subunit: Associates with the 60S ribosomal subunit of the 80S translational complex. Subcellular Location: Cytoplasm Similarity: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. Database links: Entrez Gene: 23173 Human Entrez Gene: 75624 Mouse Entrez Gene: 295500 Rat Omim: 610151 Human SwissProt: P53582 Human SwissProt: Q8BP48 Mouse Unigene: 480364 Human Unigene: 26833 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
Meta AP-1是在所有组织中低表达的394氨基酸蛋白，但在骨骼肌中高度表达。MATAP-1的活性位点含有两个相邻的二价金属离子，它们由水分子或氢氧根离子连接。哺乳动物细胞增殖的控制直接相关，严格依赖于MetAP-1所采用的进化高度保守的机制。真核生物含有Meta AP-1和Meta 2，而原核生物仅含有Meta -1酶。吡啶-2-羧酸噻唑-2-酰基酰胺(PCAT)形成抑制MetAP-1作用的支架，而1,2,4-三唑是MetAP-1结合到活性位点的非肽类抑制剂，与两个二价离子络合的三唑部分的N1和N2原子结合。