LAMP2 抗体
Applications
In-house applications |
ELISA, WB, IF, FC, IHC Application not tested? Please see our Guarantee. |
Published applications |
IF, WB |
Publications |
4 citations –view here |
Species specificity |
Human, Mouse; other species not tested. |
Published Species |
Human |
Images
Prev12Next
- L02 cells were subjected to SDS PAGE followed by western blot with 10397-1-AP(LAMP2 antibody) at dilution of 1:300
- Immunohistochemical of paraffin-embedded human lung cancer using 10397-1-AP(LAMP2 antibody) at dilution of 1:50 (under 10x lens)
- Immunohistochemical of paraffin-embedded human lung cancer using 10397-1-AP(LAMP2 antibody) at dilution of 1:50 (under 40x lens)
- Immunofluorescent analysis of HepG2 cells, using LAMP2 antibody 10397-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- 1X10^6 HepG2 cells were stained with .2ug LAMP2 antibody (10397-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Positive WB detected in |
L02 cells, HEK-293 cells, HeLa cells, HepG2 cells, HT-1080 cells, human placenta tissue, K-562 cells, mouse kidney tissue, mouse skeletal muscle tissue, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in |
Human lung cancer tissue, human colon cancer tissue, human placenta tissue, human skeletal muscle tissue |
Positive IF detected in |
HepG2 cells |
Positive FC detected in |
HepG2 cells |
Recommended dilution |
WB : 1:200-1:1000
IHC : 1:50-1:200
IF : 1:10-1:100 |
LAMP2 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source |
Rabbit |
Purification method |
Antigen affinity purification |
Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
Immunogen |
LAMP2 fusion protein ag0595 |
Full name |
Lysosomal-associated membrane protein 2 |
Calculated molecular
weight |
45 kDa |
Observed molecular
weight |
50-55 kDa |
GenBank accession
number |
BC002965 |
Gene ID (NCBI) |
3920 |
Gene symbol |
LAMP2 |
Synonyms |
CD107b, LAMP 2, LAMP2, LAMPB, LGP110 |
Background LAMP2 抗体
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides. LAMP2 抗体
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