c-fos,c-fos抗体

产品编号HZ-23042R
英文名称c-fos
中文名称c-fos抗体
别 名Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogene homolog; FOS; AP-1; C-FOS; p55; FOS_HUMAN; Proto-oncogene c-Fos; G0/G1 switch regulatory protein 7.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学 神经生物学 信号转导 转录调节因子 肿瘤细胞生物标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
c-fos,c-fos抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量41kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human c-fos
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
c-fos,c-fos抗体PubMedPubMed
产品介绍background:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Function:
Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.

Subunit:
Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activation. Interacts with MAFB. Interacts withDSIPI; this interaction inhibits the binding of active AP1 to itstarget DNA.

Subcellular Location:
Nucleus.

c-fos,c-fos抗体Post-translational modifications:
Phosphorylated in the C-terminal upon stimulation by nervegrowth factor (NGF) and epidermal growth factor (EGF).Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on bothSer-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to proteinstabilization with phosphorylation on Ser-374 being the major sitefor protein stabilization on NGF stimulation. Phosphorylation onSer-362 and Ser-374 primes further phosphorylations on Thr-325 andThr-331 through promoting docking of MAPK to the DEF domain.Phosphorylation on Thr-232, induced by HA-RAS, activates thetranscriptional activity and antagonizes sumoylation.Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes toosteoblast transformation (By similarity). [PTM] Constitutively sumoylated by SUMO1, SUMO2 and SUMO3.Desumoylated by SENP2. Sumoylation requires heterodimerization withJUN and is enhanced by mitogen stimulation. Sumoylation inhibitsthe AP-1 transcriptional activity and is, itself, inhibited byRas-activated phosphorylation on Thr-232.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

Gene ID:
2353

Database links:
Entrez Gene: 2353 Human
Entrez Gene: 14281 Mouse
Entrez Gene: 314322 Rat
Omim: 164810 Human
SwissProt: P01100 Human
SwissProt: P01101 Mouse
SwissProt: P12841 Rat
Unigene: 246513 Mouse
Unigene: 103750 Rat

c-fos,c-fos抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

c-fos的作用主要用于各种类型的恶性肿瘤如食管癌、鼻咽癌、乳腺癌、结肠癌以及脑病的研究。 c-fos原癌基因及其蛋白产物不仅参与细胞的正常生长、分化过程，而且也参与细胞内信息传递过程和细胞的能量代谢过程，对细胞的增生、分化、转化都有调节作用、在生命活动中起着极为基础而重要的作用。